Journal: STAR Protocols
Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing
doi: 10.1016/j.xpro.2026.104513
Figure Lengend Snippet: Library preparation Schematic diagram illustrating the major steps of library construction using the NEBNext Ultra II DNA Library Prep Kit. The workflow includes. (1) end repair and dA-tailing of fragmented genomic DNA. (2) adaptor ligation (hairpin adaptor containing dU); USER enzyme cleavage to open the adaptor, and (3) PCR enrichment with indexed primers to generate the final sequencing library.
Article Snippet: Recover the supernatant containing DNA that are bound by FOXP3 by using QIAquick PCR & Gel Cleanup Kit (Qiagen #28506) according to manufacturer’s instructions . b.
Techniques: Ligation, Sequencing